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KMID : 0928520090190030241
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Korean Journal of Lipidology
2009 Volume.19 No. 3 p.241 ~ p.249
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Adipose Tissue Stromal Vascular Fraction Cells Differentiate Depending on Various Methods
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Kim Min-Kyung
Park Yong-Soon
Park Hee-soon
Choi Jung-mook
Kim Won-Jun
Park Se-Eun
Park Cheol-Young
Lee Won-young
Oh Ki-won
Park Sung-woo
Kim Sun-Woo
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Abstract
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Objective: Adipogenic medium of fat cells can be induced using culture medium supplemented with insulin, 3-isobutyl-methylxanthine (IBMX), glucocorticoid and indomethacin. The purpose of this study was to determine medium conditions for promoting differentiation of stromal vascular fraction (SVF) cells towards adipocyte.
Methods: SVFs were isolated subcutaneous adipose tissues and retroperitoneal adipose tissue of Long-Evans Tokushima Otsuka (LETO) rat and Sprague-Dawley rat. Post-confluent SVFs were cultured MDI medium (520 μM 3-isobutyl-methylxanthine (IBMX), 1 μM dexamethasone, 1 μg/mL insulin) for 2 days 3 times or MDII medium (MDI medium, 200 μM indomethacin) for 3 days 3 times or MDIT medium (MDI medium, 20 μg/mL troglitazone) for 2 days.
Results: SVF cells adipogenic differentiation were higher to add the MDI medium 3 times, MDII medium or MDIT medium than add the MDI medium.
Conclusion:Adipogenic differentiation was enhanced adding the adipogenic medium multiple times and using the different PPARγ ligands (indomethacin, troglitazone).
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KEYWORD
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Stromal vascular fraction, Density gradient, Indomethacine, PPARγ agonist, Differentiation
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